P-384 TP53 protein expression in colorectal cancer

In human colorectal cancer (CRC), TP53 is one of the most important drives genes; it a key regulator apoptosis. The dysfunction gene critical event in development CRC this context, we aimed to evaluate expression Tp53 patients and establish correlation between overexpression p53 with clinical pathological features Colorectal Carcinoma (CRC). This study enrolled 32 carcinoma. was investigated by immunohistochemistry (IHC) on formalin fixed paraffin embedded sections using NCL-P53 (NCL-L-p53-DO7, Novocastra) monoclonal antibody. nuclear staining scored according percentage stained tumor cells. cutoff set at 30%. With reference score, classified into two categories: negative (= 30%). Among samples over expressed 53.1 % (17/32) samples. 41.2% left colon, 35.3% right colon 23.5% rectum. There no significant positivity gender (p = 0.64), age (< 50 vs. ≥ year; p 0.77), site (right rectum; 0.7), stade (p=0.19) RAS mutation status (p=0.58). study, protein overexpressed CRC. knowledge that immunohistochemical detection may indicate theTP53 suppressor gene, finding suggests play role carcinogenesis process.